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Bioengineering

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A Simple Hanging Drop Cell Culture Protocol for Generation of 3D Spheroids

Article DOI: 10.3791/2720-v • 07:24 min • May 6th, 2011
May 6th, 2011 •
1
1Department of Surgery, UMDNJ-Robert Wood Johnson Medical School

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We describe a simple, rapid method of generating 3D tissue-like spheroids and their potential application to quantify differences in cell-cell interactions.

Tags

Hanging Drop Cell Culture 3D Spheroids Cell-cell Cohesion Cell-substratum Adhesion Monolayer Culture Rigid Substrates Tissue Mass Intimate Connections Extracellular Matrix Components Chemical Milieu Physical Forces Cellular Morphology Signaling Collagen Gels Biomaterial Scaffolds Direct Cell-cell Adhesion Architecture Culture Systems ECM Products Physiological Conditions Biological Agent
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