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Monte todo In Situ Hibridação de E8.5 de Embriões E11.5 rato
JoVE Journal
Biologie
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JoVE Journal Biologie
Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos
DOI:

13:54 min

October 10, 2011

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Kapitel

  • 00:05Titel
  • 01:09Generation of a Gene-specific Riboprobe and Measurement of Specific Activity
  • 03:58Collection of Mouse Embryos
  • 05:12Hybridization of the Digoxygenin-labeled Probe to Whole Mouse Embryos
  • 10:07Antibody Detection of the Digoxygenin-labeled Riboprobe
  • 12:38Examples of Whole-mount in situ Hybridizations
  • 13:29Conclusion

Summary

Automatische Übersetzung

This whole mount in situ hybridization protocol discusses critical steps that ensure reproducible high quality results for gene expression studies in E8.5-E11.5 day old mouse embryos.

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