Journal
/
Biology
/
Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos

DOI:
10.3791/2797-v

•

13:54 min

•

October 10, 2011

•

, ,
1Department of Genetics,University of Georgia

Chapters

  • 00:05Title
  • 01:09Generation of a Gene-specific Riboprobe and Measurement of Specific Activity
  • 03:58Collection of Mouse Embryos
  • 05:12Hybridization of the Digoxygenin-labeled Probe to Whole Mouse Embryos
  • 10:07Antibody Detection of the Digoxygenin-labeled Riboprobe
  • 12:38Examples of Whole-mount in situ Hybridizations
  • 13:29Conclusion

Summary

Automatic Translation

Automatic Translation

This whole mount in situ hybridization protocol discusses critical steps that ensure reproducible high quality results for gene expression studies in E8.5-E11.5 day old mouse embryos.

Tags

Whole MountIn Situ HybridizationGene Expression PatternsEmbryosProbe LabelingQuality ControlDIG-labeled RNA ProbesIn Vitro TranscriptionPCRSensitivityEndogenous MRNAFixationStorageProteinase K DigestionHybridization ConditionsPost-hybridization WashesRNase TreatmentNon-specific Probe HybridizationAP-conjugated Antibody

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/2797?article_type=v

Double Whole Mount in situ Hybridization of Early Chick Embryos
check_url/2797?article_type=v

Method for Whole Mount Antibody Staining in Chick
check_url/2797?article_type=v

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
check_url/2797?article_type=v

Using Whole Mount in situ Hybridization to Link Molecular and Organismal Biology
check_url/2797?article_type=v

A High Throughput in situ Hybridization Method to Characterize mRNA Expression Patterns in the Fetal Mouse Lower Urogenital Tract
check_url/2797?article_type=v

Whole Mount RNA Fluorescent in situ Hybridization of Drosophila Embryos
check_url/2797?article_type=v

RNA In situ Hybridization in Whole Mount Embryos and Cell Histology Adapted for Marine Elasmobranchs
check_url/2797?article_type=v

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
check_url/2797?article_type=v

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function
check_url/2797?article_type=v

Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3

Read Article