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Planar Gradient système de diffusion d'enquêter Chimiotactisme dans une matrice de collagène 3D
JoVE Journal
Biotechnik
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JoVE Journal Biotechnik
Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix
DOI:

09:26 min

June 12, 2015

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Kapitel

  • 00:05Titel
  • 01:17Hydrophillic Glass Coverslips Preparation
  • 03:00Hydrophobic Glass Coverslips Preparation
  • 03:493D Silicone Mold Assembly
  • 05:43Creation of Collagen Mixture and 3D Matrix
  • 07:53Results: Chemoattractants Used in Planar Gradient Diffusion System Directs Cell Movement through Collagen Matrix
  • 09:03Conclusion

Summary

Automatische Übersetzung

Cell migration is an important part of human development and life. In order to understand the mechanisms that can alter cell migration, we present a planar gradient diffusion system to investigate chemotaxis in a 3D collagen matrix, which allows one to overcome modern diffusion chamber limitations of existing assays.

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