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Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix
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Bioengineering
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JoVE Journal Bioengineering
Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix

Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix

DOI:
10.3791/52948-v

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09:26 min

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June 12, 2015

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, ,
1Department of Mechanical and Aerospace Engineering,California State University, Long Beach, 2Ximedica, 3School of Engineering,Brown University

Chapters

  • 00:05Title
  • 01:17Hydrophillic Glass Coverslips Preparation
  • 03:00Hydrophobic Glass Coverslips Preparation
  • 03:493D Silicone Mold Assembly
  • 05:43Creation of Collagen Mixture and 3D Matrix
  • 07:53Results: Chemoattractants Used in Planar Gradient Diffusion System Directs Cell Movement through Collagen Matrix
  • 09:03Conclusion

Summary

Automatic Translation

Automatic Translation

Cell migration is an important part of human development and life. In order to understand the mechanisms that can alter cell migration, we present a planar gradient diffusion system to investigate chemotaxis in a 3D collagen matrix, which allows one to overcome modern diffusion chamber limitations of existing assays.

Tags

Keywords: Cell MigrationChemotaxis3D Collagen MatrixTraction Force MicroscopyDigital Volume CorrelationDiffusion GradientChemoattractant

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