Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Vurdering av neuronal levedyktighet ved bruk av…
JoVE Journal
Neuroscience

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Neuroscience
Vurdering av neuronal levedyktighet ved bruk av fluoresceindiacetat-propidiumjodid-dobbeltfarvning i cerebellar granulærneuronkultur
Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation
Journal (Neuroscience)
Quantifying the Activity of cis-Regulatory Elements in the Mouse Retina by Explant Electroporation
In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
Journal (Neuroscience)
In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
 
Click here for the English version

Vurdering av neuronal levedyktighet ved bruk av fluoresceindiacetat-propidiumjodid-dobbeltfarvning i cerebellar granulærneuronkultur

Article DOI: 10.3791/55442-v • 08:53 min • May 10th, 2017
May 10th, 2017 •
*1, *2, 1, 1, 1, 2, 3, 1, 2,4, 1,2
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy, Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
* These authors contributed equally

Kapitel

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Denne protokollen beskriver hvordan man nøyaktig måler neuronal levedyktighet ved å bruke fluoresceindiacetat (FDA) og propidiumjodid (PI) dobbeltfarging i dyrkede cerebellargranulæreuroner, en primær nevronkultur som brukes som en in vitro- modell i nevrovitenskapelig og neurofarmakologisk forskning.

Tags

Neurovitenskap utgave 123 cerebellar granulæreuroner fluoresceindiacetat propidiumjodid cellelevbarhet glialceller fluorescensmikroskopi
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter