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Journal / Neuroscience / 소뇌 과립 신경 세포 배양에서 Fluorescein Diacetate-Propidium…
JoVE Journal
Neuroscience

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JoVE Journal Neuroscience
소뇌 과립 신경 세포 배양에서 Fluorescein Diacetate-Propidium Iodide 이중 염색을 이용한 신경 생존력의 평가
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소뇌 과립 신경 세포 배양에서 Fluorescein Diacetate-Propidium Iodide 이중 염색을 이용한 신경 생존력의 평가

Article DOI: 10.3791/55442-v • 08:53 min • May 10th, 2017
May 10th, 2017 •
*1, *2, 1, 1, 1, 2, 3, 1, 2,4, 1,2
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy, Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
* These authors contributed equally

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이 프로토콜은 신경 과학 및 신경 약리학 연구 에서 체외 모델로 사용되는 배양 소뇌 과립 뉴런 (neuronal culture)의 Fluorescein diacetate (FDA) 및 Propidium Iodide (PI) 이중 염색을 사용하여 신경 생존력을 정확하게 측정하는 방법을 설명합니다.

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신경 과학 123 호 소뇌 과립 뉴런 플루 오시아 신 디 아세테이트 요오드화 프로피 디움 세포 생존력 신경 교세포 형광 현미경
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