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Journal / Neuroscience / Avaliação da Viabilidade Neuronal Utilizando…
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Neuroscience

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Avaliação da Viabilidade Neuronal Utilizando Coloração Dupla de Iodeto de Diacetate-Propidium de Fluoresceína em Cultura de Neurônio de Granulado Cerebelar

Article DOI: 10.3791/55442-v • 08:53 min • May 10th, 2017
May 10th, 2017 •
*1, *2, 1, 1, 1, 2, 3, 1, 2,4, 1,2
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy, Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
* These authors contributed equally

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Este protocolo descreve como medir com precisão a viabilidade neuronal usando fluoresceína diacetato (FDA) e Propidium Iodide (PI) dupla coloração em neurônios cultivados cerebrais grânulo, uma cultura primária neuronal utilizado como um modelo in vitro em neurociência e neuropharmacology investigação.

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Neuroscience Edição 123 neurônios de grânulos cerebelosos diacetato de fluoresceína iodeto de propídio viabilidade celular células gliais microscopia de fluorescência
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