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Decellularization og Recellularization metode for menneskelige Saphenous vener
JoVE Journal
Biotechnik
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JoVE Journal Biotechnik
Decellularization and Recellularization Methodology for Human Saphenous Veins

Decellularization og Recellularization metode for menneskelige Saphenous vener

DOI:
10.3791/57803-v

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11:35 min

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July 27, 2018

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, ,
1Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy,University of Gothenburg

Kapitel

  • 00:04Titel
  • 00:43Preparation of Decellularization Setups and Recellularization Bioreactor
  • 03:55Decellularization of Saphenous Vein
  • 06:14Recellularization
  • 09:22Results: Evaluation of Normal, Decellularized, and Recellularized Veins
  • 10:13Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Her, beskriver vi en protokol for saphenous vene decellularization ved hjælp af vaske-og rengøringsmidler og recellularization af perfusion af perifert blod og i endothelial medium.

Tags

DecellularizationRecellularizationSaphenous VeinTriton-X 100PerfusionWashingBioreactorCell CultureBone MarrowTnBPDNAMedia InletMedia OutletVein InletVein OutletPeristaltic Pump

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