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Journal / Biochemistry / Identification of Novel CK2 Kinase Substrates…
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Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Article DOI: 10.3791/59037-v • 11:11 min • February 21st, 2019
February 21st, 2019 •
1, 1, 1
1Department of Biochemistry and Molecular Biology, Drexel University College of Medicine

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Summary
Automatic Translation

The objective of this protocol is to label, enrich, and identify substrates of protein kinase CK2 from a complex biological sample such as a cell lysate or tissue homogenate. This method leverages unique aspects of CK2 biology for this purpose.

Tags

CK2 Kinase Substrates Biochemical Approach Labeling Enrichment Identification Endogenous Substrates Complex Biological Sample Cell Lysate Tissue Lysate CK2 Study John Chojnowski Tissue Sample Lysis Centrifugation Supernatant Transfer Input Control Sample Labeling Kinase Reaction Tube GTPgammaS Lysis Buffer PNBM Tube
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