Probenvorbereitung zur bioinformatischen Analyse der DNA-Methylierung: Assoziationsstrategie für Adipositas und verwandte Merkmalsstudien

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Zitiert von 9

14:56 Min.

6. Mai 2022

10.3791/62598-v

6. Mai 2022

6.3K Ansichten

Natália Yumi Noronha^*¹ , Guilherme da Silva Rodrigues^*¹ , Marcela Augusta de Souza Pinhel¹ , Jean-Baptiste Cazier²^,³ , Lígia Moriguchi Watanabe¹ , Albert Nobre Menezes⁴ , Carlos Roberto Bueno⁵ , Carolina Ferreira Nicoletti¹ , Bruno Affonso Parenti de Oliveira¹ , Isabelle Mello Schineider⁶ , Isabella Harumi Yonehara Noma⁷ , Igor Caetano Dias Alcarás⁸ , Fernando Barbosa⁹ , Carla Barbosa Nonino⁶

¹Department of Internal Medicine, Ribeirão Preto Medical School, University of São Paulo, ²Centre for Computational Biology, University of Birmingham, ³Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, ⁴Cancer Genetics and Evolution Laboratory, Cancer Research UK, Institute of Genetics & Molecular Medicine, The University of Edinburgh, ⁵Ribeirão Preto School of Physical Education and Sport, University of São Paulo, ⁶Health Sciences Department, Ribeirao Preto Medical School, University of Sao Paulo, ⁷Faculty of Pharmaceutical Sciences, University of São Paulo, ⁸Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, ⁹Department of Clinical, Bromatological and Toxicological Analysis, Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of São Paulo

^* These authors contributed equally

Die vorliegende Studie beschreibt den Workflow zur Verwaltung von DNA-Methylierungsdaten, die durch Microarray-Technologien erhalten werden. Das Protokoll veranschaulicht die Schritte von der Probenvorbereitung bis zur Datenanalyse. Alle Verfahren werden detailliert beschrieben, und das Video zeigt die wesentlichen Schritte.