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Journal / Biochemistry / Probing RNA Structure with Dimethyl Sulfate…
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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Article DOI: 10.3791/64820-v • 10:34 min • December 9th, 2022
December 9th, 2022 •
1, 1
1Department of Microbiology, Harvard Medical School

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Summary
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The protocol provides instruction for modifying RNA with dimethyl sulfate for mutational profiling experiments. It includes in vitro and in vivo probing with two alternative library preparation methods.

Tags

RNA Structure Dimethyl Sulfate Mutational Profiling Sequencing DMS-MaP Method Crystallography Electron Microscopy RNA Structure Ensembles Biological Research Refolding Buffer Nuclease-free Water PCR Tube Thermocycler Denature RNA Ice Block Refold RNA Dimethyl Sulfate (DMS) Concentration
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