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JOVE JOURNAL
Cancer Research
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Journal (Cancer Research)
Plating CFSE-Labelled Tumor Cells and Co-Culturing CAR-Expressing Jurkats for In Vitro Cytotoxicity Evaluation
Siddharth Subham*
1,2,3
,
John D. Jeppson*
1
,
Benjamin K. Gibbs
,
Jacqueline Babai
,
Riza Alker
,
Andrew K. Godwin
5,6
,
David Akhavan
2,3
1
Department of Radiation Oncology
,
University of Kansas Cancer Center
,
2
Department of Cancer Biology
,
University of Kansas Cancer Center
,
3
BioEngineering Program
,
University of Kansas
,
4
Department of Pathology and Laboratory Medicine
,
University of Kansas Medical Center
,
5
University of Kansas Cancer Center
,
6
Kansas Institute for Precision Medicine
,
University of Kansas Medical Center
Author
Siddharth Subham*
1,2,3
,
John D. Jeppson*
1
,
Benjamin K. Gibbs
,
Jacqueline Babai
,
Riza Alker
,
Andrew K. Godwin
5,6
,
David Akhavan
2,3
Institution
1
Department of Radiation Oncology
,
University of Kansas Cancer Center
,
2
Department of Cancer Biology
,
University of Kansas Cancer Center
,
3
BioEngineering Program
,
University of Kansas
,
4
Department of Pathology and Laboratory Medicine
,
University of Kansas Medical Center
,
5
University of Kansas Cancer Center
,
6
Kansas Institute for Precision Medicine
,
University of Kansas Medical Center
AUTHORS
Journal (Cancer Research)
Preparation of Plate for Fluorescent Imaging and Cell Viability Assessment in CAR-Jurkat Co-Culture with CFSE-Labeled Tumor Cells
Siddharth Subham*
1,2,3
,
John D. Jeppson*
1
,
Benjamin K. Gibbs
,
Jacqueline Babai
,
Riza Alker
,
Andrew K. Godwin
5,6
,
David Akhavan
2,3
1
Department of Radiation Oncology
,
University of Kansas Cancer Center
,
2
Department of Cancer Biology
,
University of Kansas Cancer Center
,
3
BioEngineering Program
,
University of Kansas
,
4
Department of Pathology and Laboratory Medicine
,
University of Kansas Medical Center
,
5
University of Kansas Cancer Center
,
6
Kansas Institute for Precision Medicine
,
University of Kansas Medical Center
Author
Siddharth Subham*
1,2,3
,
John D. Jeppson*
1
,
Benjamin K. Gibbs
,
Jacqueline Babai
,
Riza Alker
,
Andrew K. Godwin
5,6
,
David Akhavan
2,3
Institution
1
Department of Radiation Oncology
,
University of Kansas Cancer Center
,
2
Department of Cancer Biology
,
University of Kansas Cancer Center
,
3
BioEngineering Program
,
University of Kansas
,
4
Department of Pathology and Laboratory Medicine
,
University of Kansas Medical Center
,
5
University of Kansas Cancer Center
,
6
Kansas Institute for Precision Medicine
,
University of Kansas Medical Center
AUTHORS
Journal (Cancer Research)
Nuclei Isolation from Human Pancreatic Tumor Cells for Multiome Sequencing
Deshka S. Foster
,
Michelle Griffin
,
Michael Januszyk
,
Daniel Delitto
,
Jeffrey A. Norton
,
Michael T. Longaker
1
Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery
,
Stanford University
Author
Deshka S. Foster
,
Michelle Griffin
,
Michael Januszyk
,
Daniel Delitto
,
Jeffrey A. Norton
,
Michael T. Longaker
Institution
1
Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery
,
Stanford University
AUTHORS
Journal (Cancer Research)
Optimized Nuclei Isolation from Fresh and Frozen Solid Tumor Specimens for Multiome Sequencing
Deshka S. Foster
,
Michelle Griffin
,
Michael Januszyk
,
Daniel Delitto
,
Jeffrey A. Norton
,
Michael T. Longaker
1
Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery
,
Stanford University
Author
Deshka S. Foster
,
Michelle Griffin
,
Michael Januszyk
,
Daniel Delitto
,
Jeffrey A. Norton
,
Michael T. Longaker
Institution
1
Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery
,
Stanford University
AUTHORS
Journal (Cancer Research)
Stabilized Window for Intravital Imaging of the Murine Pancreas
Jakeb Petersen*
1,2,3
,
Wei Du*
4,5
,
Christian Adkisson
4
,
Claudia Gravekamp
7
,
Maja H. Oktay
3,4,7,8,9
,
John Condeelis
3,4,7,8,9,10
,
Nicole C. Panarelli
3,7
,
John C. McAuliffe
2,3,7,8
,
David Entenberg
3,4,7,8,9
1
Department of Surgery
,
Einstein College of Medicine / Montefiore Medical Center
,
2
Department of Pathology
,
Einstein College of Medicine / Montefiore Medical Center
,
3
Integrated Imaging Program for Cancer Research
,
Einstein College of Medicine / Montefiore Medical Center
,
4
Department of Anatomy and Structural Biology
,
Einstein College of Medicine / Montefiore Medical Center
,
5
Breast Center
,
Peking University People's Hospital
,
6
Department of Microbiology & Immunology
,
Einstein College of Medicine / Montefiore Medical Center
,
7
Montefiore Einstein Cancer Center
,
Einstein College of Medicine / Montefiore Medical Center
,
8
Cancer Dormancy and Tumor Microenvironment Institute
,
Albert Einstein College of Medicine / Montefiore Medical Center
,
9
Gruss-Lipper Biophotonics Center
,
Einstein College of Medicine / Montefiore Medical Center
,
10
Department of Cell Biology
,
Einstein College of Medicine / Montefiore Medical Center
Author
Jakeb Petersen*
1,2,3
,
Wei Du*
4,5
,
Christian Adkisson
4
,
Claudia Gravekamp
7
,
Maja H. Oktay
3,4,7,8,9
,
John Condeelis
3,4,7,8,9,10
,
Nicole C. Panarelli
3,7
,
John C. McAuliffe
2,3,7,8
,
David Entenberg
3,4,7,8,9
Institution
1
Department of Surgery
,
Einstein College of Medicine / Montefiore Medical Center
,
2
Department of Pathology
,
Einstein College of Medicine / Montefiore Medical Center
,
3
Integrated Imaging Program for Cancer Research
,
Einstein College of Medicine / Montefiore Medical Center
,
4
Department of Anatomy and Structural Biology
,
Einstein College of Medicine / Montefiore Medical Center
,
5
Breast Center
,
Peking University People's Hospital
,
6
Department of Microbiology & Immunology
,
Einstein College of Medicine / Montefiore Medical Center
,
7
Montefiore Einstein Cancer Center
,
Einstein College of Medicine / Montefiore Medical Center
,
8
Cancer Dormancy and Tumor Microenvironment Institute
,
Albert Einstein College of Medicine / Montefiore Medical Center
,
9
Gruss-Lipper Biophotonics Center
,
Einstein College of Medicine / Montefiore Medical Center
,
10
Department of Cell Biology
,
Einstein College of Medicine / Montefiore Medical Center
AUTHORS
Journal (Cancer Research)
Pancreas Tumor Implantation in Mice
Jakeb Petersen*
1,2,3
,
Wei Du*
4,5
,
Christian Adkisson
4
,
Claudia Gravekamp
7
,
Maja H. Oktay
3,4,7,8,9
,
John Condeelis
3,4,7,8,9,10
,
Nicole C. Panarelli
3,7
,
John C. McAuliffe
2,3,7,8
,
David Entenberg
3,4,7,8,9
1
Department of Surgery
,
Einstein College of Medicine / Montefiore Medical Center
,
2
Department of Pathology
,
Einstein College of Medicine / Montefiore Medical Center
,
3
Integrated Imaging Program for Cancer Research
,
Einstein College of Medicine / Montefiore Medical Center
,
4
Department of Anatomy and Structural Biology
,
Einstein College of Medicine / Montefiore Medical Center
,
5
Breast Center
,
Peking University People's Hospital
,
6
Department of Microbiology & Immunology
,
Einstein College of Medicine / Montefiore Medical Center
,
7
Montefiore Einstein Cancer Center
,
Einstein College of Medicine / Montefiore Medical Center
,
8
Cancer Dormancy and Tumor Microenvironment Institute
,
Albert Einstein College of Medicine / Montefiore Medical Center
,
9
Gruss-Lipper Biophotonics Center
,
Einstein College of Medicine / Montefiore Medical Center
,
10
Department of Cell Biology
,
Einstein College of Medicine / Montefiore Medical Center
Author
Jakeb Petersen*
1,2,3
,
Wei Du*
4,5
,
Christian Adkisson
4
,
Claudia Gravekamp
7
,
Maja H. Oktay
3,4,7,8,9
,
John Condeelis
3,4,7,8,9,10
,
Nicole C. Panarelli
3,7
,
John C. McAuliffe
2,3,7,8
,
David Entenberg
3,4,7,8,9
Institution
1
Department of Surgery
,
Einstein College of Medicine / Montefiore Medical Center
,
2
Department of Pathology
,
Einstein College of Medicine / Montefiore Medical Center
,
3
Integrated Imaging Program for Cancer Research
,
Einstein College of Medicine / Montefiore Medical Center
,
4
Department of Anatomy and Structural Biology
,
Einstein College of Medicine / Montefiore Medical Center
,
5
Breast Center
,
Peking University People's Hospital
,
6
Department of Microbiology & Immunology
,
Einstein College of Medicine / Montefiore Medical Center
,
7
Montefiore Einstein Cancer Center
,
Einstein College of Medicine / Montefiore Medical Center
,
8
Cancer Dormancy and Tumor Microenvironment Institute
,
Albert Einstein College of Medicine / Montefiore Medical Center
,
9
Gruss-Lipper Biophotonics Center
,
Einstein College of Medicine / Montefiore Medical Center
,
10
Department of Cell Biology
,
Einstein College of Medicine / Montefiore Medical Center
AUTHORS
Journal (Cancer Research)
Murine Pancreatic Window Surgery
Jakeb Petersen*
1,2,3
,
Wei Du*
4,5
,
Christian Adkisson
4
,
Claudia Gravekamp
7
,
Maja H. Oktay
3,4,7,8,9
,
John Condeelis
3,4,7,8,9,10
,
Nicole C. Panarelli
3,7
,
John C. McAuliffe
2,3,7,8
,
David Entenberg
3,4,7,8,9
1
Department of Surgery
,
Einstein College of Medicine / Montefiore Medical Center
,
2
Department of Pathology
,
Einstein College of Medicine / Montefiore Medical Center
,
3
Integrated Imaging Program for Cancer Research
,
Einstein College of Medicine / Montefiore Medical Center
,
4
Department of Anatomy and Structural Biology
,
Einstein College of Medicine / Montefiore Medical Center
,
5
Breast Center
,
Peking University People's Hospital
,
6
Department of Microbiology & Immunology
,
Einstein College of Medicine / Montefiore Medical Center
,
7
Montefiore Einstein Cancer Center
,
Einstein College of Medicine / Montefiore Medical Center
,
8
Cancer Dormancy and Tumor Microenvironment Institute
,
Albert Einstein College of Medicine / Montefiore Medical Center
,
9
Gruss-Lipper Biophotonics Center
,
Einstein College of Medicine / Montefiore Medical Center
,
10
Department of Cell Biology
,
Einstein College of Medicine / Montefiore Medical Center
Author
Jakeb Petersen*
1,2,3
,
Wei Du*
4,5
,
Christian Adkisson
4
,
Claudia Gravekamp
7
,
Maja H. Oktay
3,4,7,8,9
,
John Condeelis
3,4,7,8,9,10
,
Nicole C. Panarelli
3,7
,
John C. McAuliffe
2,3,7,8
,
David Entenberg
3,4,7,8,9
Institution
1
Department of Surgery
,
Einstein College of Medicine / Montefiore Medical Center
,
2
Department of Pathology
,
Einstein College of Medicine / Montefiore Medical Center
,
3
Integrated Imaging Program for Cancer Research
,
Einstein College of Medicine / Montefiore Medical Center
,
4
Department of Anatomy and Structural Biology
,
Einstein College of Medicine / Montefiore Medical Center
,
5
Breast Center
,
Peking University People's Hospital
,
6
Department of Microbiology & Immunology
,
Einstein College of Medicine / Montefiore Medical Center
,
7
Montefiore Einstein Cancer Center
,
Einstein College of Medicine / Montefiore Medical Center
,
8
Cancer Dormancy and Tumor Microenvironment Institute
,
Albert Einstein College of Medicine / Montefiore Medical Center
,
9
Gruss-Lipper Biophotonics Center
,
Einstein College of Medicine / Montefiore Medical Center
,
10
Department of Cell Biology
,
Einstein College of Medicine / Montefiore Medical Center
AUTHORS
Journal (Cancer Research)
Ovarian Cancer Patient-Derived Organoid Models for Pre-Clinical Drug Testing
Bisiayo E. Fashemi
,
Lillian van Biljon
,
Jeimmy Rodriguez
,
Olivia Graham
,
Mary Mullen
,
Dineo Khabele
1
Washington University of Medicine in St. Louis
Author
Bisiayo E. Fashemi
,
Lillian van Biljon
,
Jeimmy Rodriguez
,
Olivia Graham
,
Mary Mullen
,
Dineo Khabele
Institution
1
Washington University of Medicine in St. Louis
AUTHORS
Journal (Cancer Research)
Preparing a Vascularized Micro-Tumor System Using Human Cells for Studying Tumors and Drug Responses
Stephanie J. Hachey
,
Daniela Gaebler
,
Christopher C. W. Hughes
2
1
Molecular Biology and Biochemistry
,
University of California, Irvine
,
2
Biomedical Engineering
,
University of California, Irvine
Author
Stephanie J. Hachey
,
Daniela Gaebler
,
Christopher C. W. Hughes
2
Institution
1
Molecular Biology and Biochemistry
,
University of California, Irvine
,
2
Biomedical Engineering
,
University of California, Irvine
AUTHORS
Journal (Cancer Research)
Introducing Human Cells into a Microfluidic Device to Investigate Tumor Progression in a Vascularized Micro-Tumor System
Stephanie J. Hachey
,
Daniela Gaebler
,
Christopher C. W. Hughes
2
1
Molecular Biology and Biochemistry
,
University of California, Irvine
,
2
Biomedical Engineering
,
University of California, Irvine
Author
Stephanie J. Hachey
,
Daniela Gaebler
,
Christopher C. W. Hughes
2
Institution
1
Molecular Biology and Biochemistry
,
University of California, Irvine
,
2
Biomedical Engineering
,
University of California, Irvine
AUTHORS
Journal (Cancer Research)
Establishing a Physiologic Human Vascularized Micro-Tumor Model for Cancer Research
Stephanie J. Hachey
,
Daniela Gaebler
,
Christopher C. W. Hughes
2
1
Molecular Biology and Biochemistry
,
University of California, Irvine
,
2
Biomedical Engineering
,
University of California, Irvine
Author
Stephanie J. Hachey
,
Daniela Gaebler
,
Christopher C. W. Hughes
2
Institution
1
Molecular Biology and Biochemistry
,
University of California, Irvine
,
2
Biomedical Engineering
,
University of California, Irvine
AUTHORS
Journal (Cancer Research)
Transfection of
TMEM200A
siRNA in Stomach Adenocarcinoma Cell Lines
Yaowen Zhang
,
Shanshan Kuang
,
Hancheng Qin
,
Na Zhao
,
Yixin Yang
,
Jisheng Xie
1
Department of Histology and Embryology
,
Youjiang Medical University for Nationalities
,
2
Department of Pathophysiology
,
Youjiang Medical University for Nationalities
Author
Yaowen Zhang
,
Shanshan Kuang
,
Hancheng Qin
,
Na Zhao
,
Yixin Yang
,
Jisheng Xie
Institution
1
Department of Histology and Embryology
,
Youjiang Medical University for Nationalities
,
2
Department of Pathophysiology
,
Youjiang Medical University for Nationalities
AUTHORS
1
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8
9
10
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