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Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells
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Preparation of Plate for Fluorescent Imaging and Cell Viability Assessment in CAR-Jurkat Co-Culture with CFSE-Labeled Tumor Cells (Video) | JoVE
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Cancer Research
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JoVE Journal Cancer Research
Preparation of Plate for Fluorescent Imaging and Cell Viability Assessment in CAR-Jurkat Co-Culture with CFSE-Labeled Tumor Cells

Preparation of Plate for Fluorescent Imaging and Cell Viability Assessment in CAR-Jurkat Co-Culture with CFSE-Labeled Tumor Cells (Video) | JoVE

Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

DOI:
10.3791/200793-v

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04:00 min

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October 27, 2023

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, , , , , ,
1Department of Radiation Oncology,University of Kansas Cancer Center, 2Department of Cancer Biology,University of Kansas Cancer Center, 3BioEngineering Program,University of Kansas, 4Department of Pathology and Laboratory Medicine,University of Kansas Medical Center, 5University of Kansas Cancer Center, 6Kansas Institute for Precision Medicine,University of Kansas Medical Center

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Keywords: Fluorescent ImagingCell ViabilityCAR-Jurkat Co-cultureCFSE-labeled Tumor CellsPropidium IodideTriton XCell ImagingCFSE FluorescenceCell DiameterLive/dead Cell AnalysisCell CountingEffector-to-target RatioEGFR-targeting CARCytotoxicityPBMC-derived CD3 T Cells

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