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Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells
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Plating CFSE-Labelled Tumor Cells and Co-Culturing CAR-Expressing Jurkats for In Vitro Cytotoxicity Evaluation (Video) | JoVE
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Cancer Research
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JoVE Journal Cancer Research
Plating CFSE-Labelled Tumor Cells and Co-Culturing CAR-Expressing Jurkats for In Vitro Cytotoxicity Evaluation

Plating CFSE-Labelled Tumor Cells and Co-Culturing CAR-Expressing Jurkats for In Vitro Cytotoxicity Evaluation (Video) | JoVE

Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

DOI:
10.3791/200792-v

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03:39 min

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October 27, 2023

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, , , , , ,
1Department of Radiation Oncology,University of Kansas Cancer Center, 2Department of Cancer Biology,University of Kansas Cancer Center, 3BioEngineering Program,University of Kansas, 4Department of Pathology and Laboratory Medicine,University of Kansas Medical Center, 5University of Kansas Cancer Center, 6Kansas Institute for Precision Medicine,University of Kansas Medical Center

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Keywords: CFSE-labelingTumor CellsCAR-expressing JurkatsIn Vitro CytotoxicityCell CultureTrypsinizationCell CountingCell TransferPlate PreparationEffector-to-target Ratio

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