Viable Myeloid Cell Purification
Viable Myeloid Cell Purification - Related Videos
09:07
Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
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Cited by 27 •
2013
This protocol details a method to isolate antigen presenting cells from human thymus via different steps of enzymatic digestion of the tissue followed by density centrifugation of the single cell suspension and finally magnetic and/or FACS sorting of the cell populations of interest.
05:14
Studying Interactions between Myeloid Cells and CAR T Cells In Vitro and In Vivo
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2025
Given the limited xenograft models available to study interactions between human CART and myeloid cells, we established in vitro and in vivo models to understand the impacts of human macrophages on CART cells. Findings can potentially be generalized to evaluate macrophage roles in the tumor microenvironment and test macrophage-targeted immunotherapies.
05:26
Mechanical Dissociation: A Method to Obtain Viable Cells from a Tissue
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2023
This video describes the non-enzymatic dissociation of fresh human tissue to obtain viable cells. The technique is used for qualitative and quantitative analysis of CD45 positive cells (lymphocytes/leukocytes) present in various normal and malignant human tissues.
06:39
Culture of myeloid dendritic cells from bone marrow precursors
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Cited by 22 •
2008
This video demonstrates the procedure for differentiating myeloid dendritic cells from mouse bone marrow. Isolation of mouse tibia and femur, and processing of bone marrow are demonstrated. Pictures demonstrating cell morphology before and after differentiation, and figures depicting cell phenotype and IL-12 production following maturation using CpG are shown.
08:10
Capture and Release of Viable Circulating Tumor Cells from Blood
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Cited by 2 •
2016
A protocol to utilize a poly(N-iso-propylacrylamide) (PIPAAm) coated microfilter for effective capture and thermoresponsive release of viable circulating tumor cells (CTC) is presented. This method allows capture of CTC from patients' blood and subsequent release of viable CTC for downstream off-chip culture, analyses and characterization.
FAQs
Selective surface markers determine which cells are enriched during purification. Because monocytes, macrophages, dendritic cells, and related populations can be distinguished through their surface features, marker choice shapes the composition of the recovered fraction. This specificity allows investigators to study a defined myeloid population rather than treating all immune cells in a blood or tissue sample as equivalent.
Magnetic-activated cell sorting and fluorescence-activated cell sorting use different selection principles. The former relies on magnetic separation, whereas the latter uses fluorescence-based identification and sorting. Both can enrich selected myeloid populations, but the method chosen should match the study's need to preserve viable cells for subsequent flow cytometry, molecular profiling, functional assays, or therapeutic-response studies.
Membrane integrity and functional properties determine whether an isolated population remains biologically useful after separation. A sample may be enriched for the intended myeloid cells yet provide limited value if the cells no longer support functional or molecular analysis. Preserving these qualities enables investigators to connect cell identity with phenotype, activity, and responses measured in downstream cancer studies.
Viability makes downstream measurements more representative of living myeloid cells rather than only their detectable markers. This distinction matters when investigators evaluate immunosuppressive or inflammatory phenotypes, examine interactions with cancer cells, or assess therapeutic responses. Viable isolates therefore support interpretation of both cellular characteristics and behavior, strengthening conclusions about the tumor immune microenvironment.
The process begins with a blood or tissue sample and a defined target population, such as monocytes, macrophages, or dendritic cells. Investigators then apply selective surface markers through magnetic-activated cell sorting or fluorescence-activated cell sorting to enrich the desired cells. The resulting viable isolate can proceed to flow cytometry, molecular profiling, functional assays, or response evaluation.
This approach is useful when researchers need to characterize the tumor immune microenvironment or separate myeloid populations for focused study. Purified cells can support analysis of immunosuppressive and inflammatory phenotypes, as well as investigations of myeloid interactions with cancer cells. The method also enables evaluation of how these populations relate to therapeutic responses.
Purified viable cells can provide several complementary forms of evidence. Flow cytometry helps examine cellular populations, molecular profiling characterizes associated molecular features, and functional assays assess biological behavior. Investigators can also evaluate therapeutic responses using the isolated cells. Together, these outcomes connect myeloid-cell composition with phenotype, function, and cancer-related immune activity.