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Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
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Use of the Protease Fluorescent Detection Kit to Determine Protease Activity

Article DOI: 10.3791/1514-v • 09:00 min • August 4th, 2009
August 4th, 2009 •
1
1Sigma Aldrich

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The Protease Fluorescent Detection Kit is designed for the measurement of protease activity using fluorometry. It is also suitable for detection of trace amounts of protease contamination. The method is based on the proteolytic hydroysis of a proprietary formulation of a FITC-labeled casein substrate.

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Protease Fluorescent Detection Kit Protease Activity Assay Casein Substrate Fluorescein Isothiocyanate (FITC) Cleavage Acidification Trichloroacetic Acid (TCA) Centrifugation Pellet Acid Soluble Fragments Supernatant Neutralization Fluorescence Measurement Trypsin Protease Control Concentration Incubation Time Microcentrifuge Tubes Cuvettes Multiwell Plates
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