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JoVE Journal Biology
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Isolation and Animal Serum Free Expansion of Human Umbilical Cord Derived Mesenchymal Stromal Cells (MSCs) and Endothelial Colony Forming Progenitor Cells (ECFCs)

Article DOI: 10.3791/1525-v • 16:05 min • October 8th, 2009
October 8th, 2009 •
1, 1
1Stem Cell Research Unit, Medical University of Graz, Austria

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This protocol describes the isolation and subsequent expansion of mesenchymal stromal cells and endothelial colony forming cells without the use of animal serum to generate autologous pairs for experimental transplantation purposes.

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Isolation Animal Serum-free Human Umbilical Cord Mesenchymal Stromal Cells MSCs Endothelial Colony Forming Progenitor Cells ECFCs Progenitor Cells Regenerative Processes Tumor Formation Comparative Study Same Donor Regenerative Purposes Cellular Therapeutics Bench To Bedside Method Development Pooled Human Platelet Lysate PHPL Fetal Bovine Serum Replacement
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