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ReAsH / Flash Labeling en beeldanalyse van Tetracysteine ​​Sensor eiwitten in cellen

Article DOI: 10.3791/2857-v • 12:20 min • August 31st, 2011
August 31st, 2011 •
1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute

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De biarsenical kleurstoffen Flash en ReAsH binden specifiek aan tetracysteine ​​motieven in eiwitten en kan selectief label eiwitten in levende cellen. Onlangs is dit de etikettering strategie is gebruikt om sensoren voor verschillende eiwitten conformaties of oligomere staten te ontwikkelen. Beschrijven we de etikettering van aanpak en methoden om kwantitatief te analyseren bindend.

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Celbiologie tetracysteine TC ReAsH Flash biarsenical kleurstoffen fluorescentie imaging confocale microscopie ImageJ GFP
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