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Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

Article DOI: 10.3791/53563-v • 14:32 min • February 27th, 2016
February 27th, 2016 •
*1, *2, 1, 1
1Laboratory for Molecular Cancer Biology, Department of Radiation Oncology, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, 2Department of Radiation Oncology, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University
* These authors contributed equally

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This protocol describes the required steps to execute in vitro and in vivo deacetylation assays in order to establish the role of proteins as specific deacetylation substrates for sirtuins and further study the role of reversible - lysine acetylation as a post-translational modification.

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Deacetylation Assays Sirtuins Protein-substrates In Vitro In Vivo Acetylation Deacetylase Sirtuin 2-specific Substrate HEK 293 T Cells Lentivector GFP FLAG-tagged SIRT2 Packaging Vector Envelope Vector Polyethylenimine (PEI) Transfection Supernatant
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