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Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

Article DOI: 10.3791/54611-v • 10:13 min • November 12th, 2016
November 12th, 2016 •
1,2, 3, 1,3,4
1Center for Applied Plant Sciences, The Ohio State University, OARDC, 2Department of Plant Pathology, The Ohio State University, OARDC, 3Department of Horticulture and Crop Science, The Ohio State University, OARDC, 4OARDC Metabolite Analysis Cluster, The Ohio State University, OARDC

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This article presents a method for the detection and quantification of organic acids from plant material using free zonal capillary electrophoresis. An example of the potential application of this method, determining the effects of a secondary fermentation on organic acid levels in coffee seeds, is provided.

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Capillary Electrophoresis Quantify Organic Acids Plant Tissue Coffea Arabica Seeds Biochemistry Physiology Environmental Stimuli Root Growth Crop Development Food Quality Beverage Quality Sample Preparation High Throughput Cost-effective Mass Spectrometry Food Science Flavor Safety Stability SCCA Extraction Particle Size Liquid Nitrogen Mortar And Pestle Grinding
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