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JoVE Journal Developmental Biology
Generation of Genetically Modified Mice through the Microinjection of Oocytes
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Generation of Genetically Modified Mice through the Microinjection of Oocytes

Article DOI: 10.3791/55765-v • 10:19 min • June 15th, 2017
June 15th, 2017 •
1, 1
1Transgenic Animal Unit, Mark Wainwright Analytical Centre, University of New South Wales

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The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping strategies.

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Genetically Modified Mice Microinjection Oocytes Mouse Genesis Gene Targeting Single Guide RNA PCR Purification Kit PCR Product Binding Buffer Wash Buffer Centrifuge
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