Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / Real-Time Monitoring of Human Glioma Cell…
JoVE Journal
Cancer Research

Se requiere una suscripción a JoVE para ver este contenido.

JoVE Journal Cancer Research
Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures
Synthesis and Characterization of Placental Chondroitin Sulfate A (plCSA)-Targeting Lipid-Polymer Nanoparticles
Journal (Cancer Research)
Synthesis and Characterization of Placental Chondroitin Sulfate A (plCSA)-Targeting Lipid-Polymer Nanoparticles
Real-Time Detection and Capture of Invasive Cell Subpopulations from Co-Cultures
Journal (Cancer Research)
Real-Time Detection and Capture of Invasive Cell Subpopulations from Co-Cultures
 

Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures

Article DOI: 10.3791/59744-v • 06:51 min • December 13th, 2019
December 13th, 2019 •
1, 2, 1,3
1Molecular Neuro-oncology Laboratory, Brown University, Rhode Island Hospital, 2University of Minnesota, College of Veterinary Medicine, 3Department of Neurosurgery, Brown University

Capítulos

Resumen
Automatic Translation

Here we present an ex-vivo mixed monolayer culture system for the study of human glioma cell (hGC) migration in real-time. This model provides the ability to observe interactions between hGCs and both myelinated and non-myelinated axons within a compartmentalized chamber.

Tags

Real-time Monitoring Human Glioma Cell Migration Dorsal Root Ganglion Axon-oligodendrocyte Co-cultures Ex Vivo Co-culture System Cellular Mechanisms Molecular Mechanisms In Vitro Drug Efficacy Testing Human Glioblastoma Cells Axons John Zepecki Graduate Student Compartmented Culture Dishes Collagen Stock Solution Sterile Distilled Water Polymerize The Collagen Laminar Flow Hood Concentrated Ammonium Hydroxide Silicone Grease
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter