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Mekaniken i (poro-)elastiska kontraktila aktomyosinnätverk som modellsystem för cellcytoskelettet
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Mekaniken i (poro-)elastiska kontraktila aktomyosinnätverk som modellsystem för cellcytoskelettet

Article DOI: 10.3791/64377-v • 08:50 min • March 10th, 2023
March 10th, 2023 •
1, 1, 1, 1,2
1Department of Chemical Engineering, Ben Gurion University of the Negev, 2Department of Chemical Engineering, Ilse Kats Institute for Nanoscale Science and Technology, Ben Gurion University of the Negev

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I detta arbete används en in vitro-rekonstitueringsmetod för att studera poroelasticiteten hos aktomyosingeler under kontrollerade förhållanden. Dynamiken hos aktomyosingelen och det inbäddade lösningsmedlet kvantifieras, genom vilket nätverksporoelasticiteten demonstreras. Vi diskuterar också experimentella utmaningar, vanliga fallgropar och relevans för cellcytoskelettmekanik.

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Bioteknik utgåva 193 aktin myosin aktiva kontraktila nätverk aktomyosin poroelasticitet vätskeflöde cytoskelett-cytosolhastighetskorrelation
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