Name: Microdisección multinivel y perfil funcional-estructural de ramas arteriales renales humanas (Video) | JoVE Journal
Uploaded: 2025-09-05T13:00:00.000+00:00
Duration: 6 min 51 s
Description: Aprenda a realizar microdisección de ramas arteriales renales humanas y evaluación funcional mediante miografía de alambre. Este protocolo permite analizar propiedades vasculares específicas de humanos para desarrollo de fármacos.

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</ol>

<table><tbody><tr><td>Cloruro de acetilcolina</td><td>Sigma-Aldrich, Merck, Darmstadt, Alemania</td><td>Referencia A6625</td><td></td></tr><tr><td>Plato de cultivo de fondo negro</td><td>Danish Myo Technology, Aarhus, Dinamarca</td><td>300412</td><td></td></tr><tr><td>CaCl2</td><td>Sangon Biotech Co., Ltd., Shanghái, China</td><td>A501330</td><td></td></tr><tr><td>D-glucosa</td><td>Sangon Biotech Co., Ltd., Shanghái, China</td><td>A610219</td><td></td></tr><tr><td>Pinzas Dumont</td><td>Danish Myo Technology, Aarhus, Dinamarca</td><td>300413</td><td></td></tr><tr><td>Tijera de disección Geuder</td><td>Danish Myo Technology, Aarhus, Dinamarca</td><td>400431</td><td></td></tr><tr><td>Alambre guía</td><td>Danish Myo Technology, Aarhus, Dinamarca</td><td>400447</td><td>Diámetro 40 &micro; m</td></tr><tr><td>Kcl</td><td>Sangon Biotech Co., Ltd., Shanghái, China</td><td>A100395</td><td></td></tr><tr><td>KH2PO4</td><td>Sangon Biotech Co., Ltd., Shanghái, China</td><td>A100781</td><td></td></tr><tr><td>LabChart Professional versión 8.3&nbsp;</td><td>ADInstruments, Australia</td><td>-</td><td></td></tr><tr><td>MgCl2&middot; 6H2O</td><td>Sangon Biotech Co., Ltd., Shanghái, China</td><td>A100288</td><td></td></tr><tr><td>Sistema de multimiógrafo</td><td>Danish Myo Technology, Aarhus, Dinamarca</td><td>620 millones</td><td></td></tr><tr><td>NaCl</td><td>Sangon Biotech Co., Ltd., Shanghái, China</td><td>A100241</td><td></td></tr><tr><td>NaHCO3</td><td>Sangon Biotech Co., Ltd., Shanghái, China</td><td>A100865</td><td></td></tr><tr><td>Fenilefrina</td><td>Sigma-Aldrich, Merck, Darmstadt, Alemania</td><td>P6126</td><td></td></tr></tbody></table>

microdisección multinivel y perfil funcional-estructural de ramas arteriales renales humanas

Multilevel Microdissection and Functional-Structural Profiling of Human Renal Arterial Branches

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La arteria arqueada se...

Microdisección multinivel y perfil funcional-estructural de ramas arteriales renales humanas

Renal vascular dysfunction plays a critical role in the pathogenesis of multiple clinical conditions, including acute kidney injury, renal ischemia, and ...

multilevel-microdissection-functional-structural-profiling-human

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El riñón adultos de pez cebra es un sistema excelente para estudios de regeneración y enfermedad renal. Un aspecto esencial de este tipo de investigación es la evaluación de la estructura y función de nefronas. Este protocolo describe varios métodos que se pueden aplicar para evaluar la composición de túbulos nefrona y para evaluar la reabsorción renal.

Análisis de Composición Nephron y función en el riñón adultos de pez cebra

Se describe el uso de la resonancia magnética de campo ultra alto como una forma no invasiva de obtener información fenotípica de modelos de roedores para la enfermedad renal poliquística y para monitorear las intervenciones. En comparación con el enfoque histológico tradicional, las imágenes de resonancia magnética se pueden adquirir in vivo, lo que permite un seguimiento longitudinal.

El uso de ultra-alta MRI Field en Modelos pequeño roedor de la enfermedad renal poliquística para<em&gt; En Vivo</em&gt; Fenotipificación de Drogas y Vigilancia

Este protocolo describe un método para aislar y cultivar rudimentos metanefricos de embriones de ratón.

Disección y cultivo de riñón embrionario de ratón

Ingeniería de tejido renales construcciones proporcionan una solución para la escasez de órganos y los efectos deletéreos de la diálisis. Aquí, describimos un protocolo micro disecar riñones murinos para el aislamiento de segmentos córtico-medular. Estos segmentos están implantados en construcciones celulares libre de andamio, formando organoides renal.

Microdisección de segmentos de tejido Renal primaria y la incorporación con la tecnología de construcción de andamio-libre nuevo

Se desarrolló un modelo modificado de ratón Goldblatt de 2 riñones 1 clip (2K1C) utilizando tubos de poliuretano para iniciar la estenosis de la arteria renal, induciendo un aumento en la expresión de renina y lesión renal. Aquí, describimos un procedimiento detallado de preparación y colocación del manguito en la arteria renal para generar un modelo de ratón 2K1C reproducible y consistente.

Un modelo modificado de dos riñones y un ratón clip de regulación de renina en la estenosis de la arteria renal

Describimos un protocolo para la microdisección láser de subsegmentos del riñón humano, incluyendo el glomérulo, tubérculo proximal, extremidad ascendente gruesa, conducto coleccionista e intersticio. A continuación, el ARN se aísla de los compartimentos obtenidos y se lleva a cabo la secuenciación de ARN para determinar los cambios en la firma transcriptómica dentro de cada subsegmento.

Aplicación de microdisección láser para descubrir transcriptómica regional en tejido renal humano

El protocolo describe el aislamiento, el cultivo y el perfil de las células endoteliales de la arteria mesentérica humana. Además, se proporciona un método para preparar la arteria humana para la transcriptómica espacial. La proteómica, la transcriptómica y los ensayos funcionales se pueden realizar en células aisladas. Este protocolo se puede reutilizar para cualquier arteria de tamaño mediano o grande.

Aislamiento y perfil de células endoteliales arteriales mesentéricas primarias humanas a nivel de transcriptoma

Aquí, presentamos un protocolo utilizando microscopía de 2 fotones en ratas Munich Wistar Fromter con glomérulos de superficie para cuantificar los efectos de la obstrucción ureteral prolongada en la dinámica y función glomerular.

Uso de microscopía de 2 fotones para cuantificar los efectos de la obstrucción ureteral unilateral crónica en los procesos glomerulares

Aquí, presentamos un protocolo detallado para el análisis proteómico de todo el riñón, túbulos corticales aislados y proteomas medulares. El estudio también compara los proteomas regionales en un modelo de ratón diabético y ratones no diabéticos.

Análisis proteómico comparativo del riñón entero, la médula y los túbulos corticales en la patogénesis diabética de la lesión renal en ratones

Aprenda a realizar microdisección de ramas arteriales renales humanas y evaluación funcional mediante miografía de alambre. Este protocolo permite analizar propiedades vasculares específicas de humanos para desarrollo de fármacos.

Microdisección multinivel y perfil funcional-estructural de ramas arteriales renales humanas

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