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JoVE Journal
Biology

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Protocol for RNAi Assays in Adult Mosquitoes (A. gambiae)
 

Protocol for RNAi Assays in Adult Mosquitoes (A. gambiae)

Article DOI: 10.3791/230-v 11:49 min July 4th, 2007
July 4th, 2007

Chapitres

résumé

Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the Dimopoulos lab to inject dsRNA into Anopheles gambiae mosquitoes, which harbor the malaria parasite. The technique manipulating the injection setup and injecting dsRNA into the thorax is illustrated.

Tags

RNAi Assays Adult Mosquitoes A. Gambiae Reverse Genetics Vector Pathogens Resistance Genes Dimopoulos Lab DsRNA Injection Anopheles Gambiae Malaria Parasite Injection Setup
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