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A Standardized Method for the Analysis of Liver Sinusoidal Endothelial Cells and Their Fenestrations by Scanning Electron Microscopy

Article DOI: 10.3791/52698-v • 08:38 min • April 30th, 2015
April 30th, 2015 •
*1,2,3, *1,2, 1,2,3, 1,2,3
1Centre for Education and Research on Ageing & ANZAC Research Institute, University of Sydney and Concord Hospital, 2Ageing and Alzheimers Institute, Concord Hospital, 3Charles Perkins Centre, University of Sydney
* These authors contributed equally

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résumé
Automatic Translation

The fenestrated liver sinusoidal endothelial cell is a biologically important filter system that is highly influenced by various diseases, toxins, and physiological states. These changes significantly impact on liver function. We describe methods for the standardisation of the measurement of the size and number of fenestrations in these cells.

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Liver Sinusoidal Endothelial Cells Fenestrations Scanning Electron Microscopy Analysis Size Number Morphology Disease Toxic Physiological States Liver Function Measurement Fixation Processing Techniques Reproducible Specimen Preparation Interpretation Perfusion Fixation Dehydration Scanning Electron Microscope Image Analysis
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