Intégration de Wet and Processes Banc sec Optimise ciblés Séquençage de nouvelle génération de faible qualité et à faible quantité biopsies de tumeurs
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April 11, 2016
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Summary
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An integrated system for targeted next-generation sequencing of oncology specimens is described. This cross-platform system is optimized for low-quality and low-quantity tumor biopsies, accommodates low DNA inputs, includes well-characterized multi-variant controls, and features a novel variant caller that is informed by quantitative pre-analytical quality control measures.
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Houghton, J., Hadd, A. G., Zeigler, R., Haynes, B. C., Latham, G. J. Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies. J. Vis. Exp. (110), e53836, doi:10.3791/53836 (2016).
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