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Injection of Syngeneic Murine Melanoma Cells to Determine Their Metastatic Potential in the Lungs

Article DOI: 10.3791/54039-v • 07:31 min • May 24th, 2016
May 24th, 2016 •
1, 2, 1
1Brain Tumor Center & Neuro-Oncology Unit, Department of Neurology, Beth Israel Deaconess Medical Center & Harvard Medical School, 2Division of Cancer Genetics, Department of Medicine, Beth Israel Deaconess Medical Center & Harvard Medical School

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Metastasis plays a profound role in the virulence of cancer, accounting for an estimated 90% of deaths. We report a protocol for a metastatic melanoma model in mice that is useful for determining the efficacy of therapeutic agents against this clinical phenomenon.

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Injection Syngeneic Murine Melanoma Cells Metastatic Potential Lungs Immunotherapy Investigative Agents Immune Response B16-BL6 Melanoma Cells Trypsin EDTA Sterile Hood RPMI Medium Motorized Pipette Cell Density Centrifuge
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