Observation et la quantification des télomères et des séquences répétitives utilisant Fluorescence<em> In Situ</em> Hybridation (FISH) avec PNA Sondes en<em> Caenorhabditis elegans</em
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August 04, 2016
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We report a concise procedure of fluorescence in situ hybridization (FISH) in the gonad and embryos of Caenorhabditis elegans for observing and quantifying repetitive sequences. We successfully observed and quantified two different repetitive sequences, telomere repeats and template of alternative lengthening of telomeres (TALT).
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Seo, B., Lee, J. Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization (FISH) with PNA Probes in Caenorhabditis elegans. J. Vis. Exp. (114), e54224, doi:10.3791/54224 (2016).
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