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Chip-based Three-dimensional Cell Culture in Perfused Micro-bioreactors

Article DOI: 10.3791/564-v • 12:40 min • May 21st, 2008
May 21st, 2008 •
1, 1, 1, 1, 1, 1
1Institute for Biological Interfaces, Forschungszentrum Karlsruhe

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We describe a chip-based platform for the three-dimensional cultivation of cells in micro-bioreactors. One chip can house up to 10 Mio. cells that can be cultivated under precisely defined conditions with regard to fluid flow, oxygen tension etc. in a sterile, closed circulation loop.

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Chip-based Three-dimensional Cell Culture Perfused Micro-bioreactors Chip Non-biodegradable Polymers Bio-degradable Polymers Dimensions Cubic Micro-containers Round Recesses Scaffold Cells Nutrient Supply Gas Supply Bioreactor Housing Closed Steril Circulation Loop Roller Pump Medium Reservoir Perfusion Superfusion Mixed Operation Mode Cell Lines Primary Cells Organotypic Functions Hepatocellular Carcinoma Cell Lines Liver Specific Genes
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