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Eksperimentell protokoll for å oppdage mitokondriefunksjon i hepatocytter utsatt for organochlorine plantevernmidler
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1State Key Laboratory of Reproductive Medicine, Institute of Toxicology,Nanjing Medical University, 2Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health,Nanjing Medical University, 3Center of Gallbladder Disease, Shanghai East Hospital, Institute of Gallstone Disease,Tongji University School of Medicine
Chapitres
- 00:04Titre
- 00:36Mitochondrial Fluorescence Intensity and Cellular ATP Detection
- 02:45Mitochondrial Membrane Potential Measurements
- 04:39Oxygen Consumption Rate (OCR) Measurements
- 06:22Results: Evaluation of Mitochondrial Function in Hepatocytes Exposed to β-HCH
- 07:43Conclusion
Å forstå påvirkningen av miljøorganochlorine plantevernmidler (OCPs) på mitokondriefunksjon i hepatocytter er viktig for å utforske mekanismen for OCPs forårsaker metabolske forstyrrelser. Dette papiret presenterer detaljerte metoder for å oppdage lever mitokondriefunksjon.
