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JoVE Journal Biology
Isolering af entero Glial celler fra Submucosa og Lamina Propria af voksen mus
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Isolering af entero Glial celler fra Submucosa og Lamina Propria af voksen mus

Article DOI: 10.3791/57629-v • 07:53 min • August 15th, 2018
August 15th, 2018 •
1,4, 1, 1, 1, 1, 3, 1,2, 1,2,5
1Department of Internal Medicine-Gastroenterology, University of Michigan, 2Department of Molecular and Integrative Physiology, University of Michigan, 3Department of Molecular, Cellular and Developmental Biology, University of Michigan, 4Department of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical University, 5Division of Gastroenterology, University of Arizona College of Medicine

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Her beskriver vi isolering af entero-glial celler fra den tarm-submucosa bruge sekventielle EDTA inkubationer til Chelat divalent kationer og derefter inkubation i ikke-enzymatiske celle opsving løsning. Plating den resulterende cellesuspension på poly-D-Lysin og laminin resulterer i en yderst højberiget kultur af submukøse glial celler for funktionelle analyse.

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Biologi spørgsmålet 138 duodenum hGFAP-Cre tdTomato EDTA kelation EGCs Ca2 + flux Sox10 GFAP
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