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Isolering av innstigning gliacellene fra Submucosa og Lamina Propria voksen museklikk
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Isolering av innstigning gliacellene fra Submucosa og Lamina Propria voksen museklikk

Article DOI: 10.3791/57629-v • 07:53 min • August 15th, 2018
August 15th, 2018 •
1,4, 1, 1, 1, 1, 3, 1,2, 1,2,5
1Department of Internal Medicine-Gastroenterology, University of Michigan, 2Department of Molecular and Integrative Physiology, University of Michigan, 3Department of Molecular, Cellular and Developmental Biology, University of Michigan, 4Department of Gastrointestinal Surgery, The First Affiliated Hospital of Guangxi Medical University, 5Division of Gastroenterology, University of Arizona College of Medicine

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Her beskriver vi isolering av enteric-gliacellene fra intestinal submucosa bruke sekvensiell EDTA incubations for å chelate divalent kasjoner og inkubasjon i ikke-enzymatisk celle gjenopprettingsløsning. Kontaktflate resulterende celle suspensjon på poly-D-lysine og laminin resulterer i en høyanriket kultur av submucosal gliacellene for funksjonsanalyse.

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Biologi problemet 138 tolvfingertarmen hGFAP-grobunn tdTomato EDTA chelation EGCs Ca2 + forandring Sox10 GFAP
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