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Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

Article DOI: 10.3791/57950-v • 04:41 min • July 3rd, 2018
July 3rd, 2018 •
1, 1,2, 3, 1, 1, 1, 1, 1,4, 1
1Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine, University of Freiburg, 2Department of Medicine, Monash University, 3Department of Medicine I, Lighthouse Core Facility, Medical Center, University of Freiburg, 4Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital, University of Cologne

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Circulating microRNAs have shown promise as biomarkers for cardiovascular diseases and acute myocardial infarctions. In this study, we describe a protocol for miRNA extraction, reverse transcription, and digital PCR for the absolute quantification of miRNAs in the serum of patients with cardiovascular disease.

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Digital PCR Quantifying Circulating MicroRNAs Acute Myocardial Infarction Cardiovascular Disease Biomarkers Quantification Real-time PCR Droplets Standard Curve Serum Samples Reverse Transcription Complementary DNA DPCR Master Mix RNA Extraction
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