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Neuroscience

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JoVE Journal Neuroscience
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Omvandling av mänskliga inducerade pluripotenta stamceller (iPSCs) till funktionella spinal och kranial motor neuron använda PiggyBac vektorer

Article DOI: 10.3791/59321-v • 07:33 min • May 1st, 2019
May 1st, 2019 •
1, 2, 2, 2, 3, 2,4, 2,4, 1,2
1Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Italy, 2Center for Life Nano Science, Istituto Italiano di Tecnologia, Italy, 3Laboratory of Stem Cell biology and Molecular Embryology, The Rockefeller University, USA, 4Department of Physiology and Pharmacology, Sapienza University of Rome, Italy

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Detta protokoll möjliggör snabb och effektiv omvandling av inducerade pluripotenta stamceller till motoriska nerv celler med en spinal eller kranial identitet, genom ektopisk uttryck av transkriptionsfaktorer från inducerbara piggyBac vektorer.

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Neurovetenskap inducerade pluripotenta stamceller motoriska nerv celler piggyBac differentiering transkriptionsfaktorer Phox2a Isl1 Ngn2 Lhx3
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