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Neuroscience

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JoVE Journal Neuroscience
Omdannelse af humane induceret pluripotente stamceller (iPSCs) til funktionel spinal og kraniel motor neuroner brug PiggyBac vektorer
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Omdannelse af humane induceret pluripotente stamceller (iPSCs) til funktionel spinal og kraniel motor neuroner brug PiggyBac vektorer

Article DOI: 10.3791/59321-v • 07:33 min • May 1st, 2019
May 1st, 2019 •
1, 2, 2, 2, 3, 2,4, 2,4, 1,2
1Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Italy, 2Center for Life Nano Science, Istituto Italiano di Tecnologia, Italy, 3Laboratory of Stem Cell biology and Molecular Embryology, The Rockefeller University, USA, 4Department of Physiology and Pharmacology, Sapienza University of Rome, Italy

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Denne protokol giver mulighed for hurtig og effektiv omdannelse af inducerede pluripotente stamceller til motoriske neuroner med en spinal eller kranie identitet, ved Ektopisk udtryk af transkription faktorer fra inducerble piggybac vektorer.

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Neurovidenskab induceret pluripotente stamceller motor neuroner piggyBac differentiering transkription faktorer Phox2a Isl1 Ngn2 Lhx3
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