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Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the Rodent Brain

Article DOI: 10.3791/59685-v • 06:04 min • May 23rd, 2019
May 23rd, 2019 •
1, 1, 2, 1
1Department of Psychological and Brain Sciences, University of Delaware, 2Plant and Soil Sciences, Delaware Biotechnology Institute, University of Delaware

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Here, we present a protocol that uses near-infrared dyes in conjunction with immunohistochemistry and high-resolution scanning to assay proteins in brain regions.

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Near-infrared Fluorescence High-resolution Scanning Protein Expression Rodent Brain Quantification Two Proteins Brain Region Molecular Signaling Pathways Pan And Phosphoproteins Cognitive Phenomena Immunohistochemistry AMPA/NMDA Ratio Cryostat Minus 9 Degrees Celsius Minus 12 Degrees Celsius Slice Isolation Frozen Rat Brain Glass Slides Immunocytochemistry Paraformaldehyde PBS Tissue Fixation TBS Cell Membranes Permeabilization Detergent Incubation Primary Antibody
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