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Biochemistry

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JoVE Journal Biochemistry
Visualisere Surface T-cell reseptor Dynamics firedimensjonalt ved hjelp av gitter lysark mikroskopi
An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
Journal (Biochemistry)
An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
Journal (Biochemistry)
Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein
 
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Visualisere Surface T-cell reseptor Dynamics firedimensjonalt ved hjelp av gitter lysark mikroskopi

Article DOI: 10.3791/59914-v • 09:24 min • January 30th, 2020
January 30th, 2020 •
1, 1,2
1Committee on Cancer Biology, The University of Chicago, 2Pritzker School of Molecular Engineering, The University of Chicago

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Målet med denne protokollen er å vise hvordan du bruker Lattice Light-Sheet Microscopy til firedimensjonalt visualisere overflatereseptordynamikk i levende celler. Her vises T-cellereseptorer på CD4+ primære T-celler.

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Biokjemi Utgave 155 gitter lysark mikroskopi immunologi T-celle reseptor bildebehandling sporing dynamikk
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