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Bruke mikrofluidikk og fluorescensmikroskopi til å studere monteringsdynamikken til enkelt actinfilamenter og bunter

Article DOI: 10.3791/63891-v • 08:02 min • May 5th, 2022
May 5th, 2022 •
1, 1, 1, 1, 1
1Université Paris Cité, CNRS, Institut Jacques Monod

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Vi presenterer protokoller for enkle aktinfilamentmikrofluidiske analyser, i kombinasjon med fluorescensmikroskopi, som gjør det mulig for en å nøyaktig overvåke individuelle aktinfilamenter i sanntid mens man sekvensielt utsetter dem for forskjellige proteinløsninger.

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