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Biochemistry

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Verifiserer strukturen og dynamikken i Nucleosomes med Atomic Force mikroskopi Imaging

Article DOI: 10.3791/58820-v • 09:52 min • January 31st, 2019
January 31st, 2019 •
1, 1, 1, 1
1Department of Pharmaceutical Sciences, University of Nebraska Medical Center

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Her presenterer vi en protokoll for å karakterisere nucleosome partikler på enkelt-molekylet nivå bruker statisk og time-lapse atomic force mikroskopi (AFM) imaging teknikker. Overflate functionalization metoden beskrevet tillater for fangst av strukturen og dynamikken i nucleosomes i høy oppløsning på nanoskala.

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Biokjemi problemet 143 AFM time-lapse nucleosome chromatin dynamikk struktur histones enkelt - molekylet DNA epigenetics imaging
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