JoVE Journal
Genetics
Genetics
Un abonnement à JoVE est nécessaire pour afficher ce contenu.
Chapitres
résumé
The bipartite GAL4-UAS system is a versatile tool for modification of gene expression in a controlled spatiotemporal manner which permits functional genetic analysis in Anopheles gambiae. The procedures described for using this system are a semi-standardized cloning strategy, sexing and screening of pupae for fluorescent protein markers and embryo fixation.