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Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node
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Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node

Article DOI: 10.3791/62236-v • 08:43 min • May 7th, 2021
May 7th, 2021 •
1,2,3, 1, 1,3, 1, 1,2,3, 1,2,3, 1,2,3
1University Hospital Munich, Department of Medicine I, Ludwig-Maximilian-Unversity Munich (LMU), 2Insitute of Surgical Research at the Walter Brendel Center of Experimental Medicine, University Hospital Munich, Ludwig-Maximilians-University (LMU), 3German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA)

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résumé
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The protocol presented here provides a step-by-step approach for the isolation of cardiac resident macrophages from the sinoatrial node (SAN) and atrioventricular node (AVN) region of mouse hearts.

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Isolation Culture Resident Cardiac Macrophages Murine Sinoatrial Node Atrioventricular Node Microdissection Fluorescence-activated Cell Sorting Sinus Node AV Node Magnetic Sorting High Purity Phenotype Region-specific Impact Cardiac Electrophysiology One-time PBS Dissecting Microscope Cardiac Anatomical Landmarks Aorta Pulmonary Artery Coronary Sinus Left Ventricle Right Ventricle Atria LV Free Wall LAA RV Free Wall
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