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Optimization of the Retinal Vein Occlusion Mouse Model to Limit Variability

Article DOI: 10.3791/62980-v • 07:23 min • August 6th, 2021
August 6th, 2021 •
*1, *2, 1, 1, 1,3,4
1Department of Pathology & Cell Biology; Vagelos College of Physicians and Surgeons, Columbia University, 2Department of Molecular Pharmacology and Therapeutics; Vagelos College of Physicians and Surgeons, Columbia University, 3Department of Neurology; Vagelos College of Physicians and Surgeons, Columbia University, 4The Taub Institute for Research on Alzheimer’s Disease and the Aging Brain; Vagelos College of Physicians and Surgeons, Columbia University
* These authors contributed equally

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Automatic Translation

Here, we describe an optimized protocol for retinal vein occlusion using rose bengal and a laser-guided retinal imaging microscope system with recommendations to maximize its reproducibility in genetically modified strains.

Tags

Retinal Vein Occlusion Mouse Model Vascular Injury Edema Development Non-invasive Technique Live Monitoring Surgical Intervention Treatment Testing Tail Veining Animal Accommodation Fiber Optic Cable Laser Control Box Retinal Imaging Microscope Laser Power Power Meter Foot Switch Pedal Experimental Laser Power
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