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JoVE Journal Genetics
Udvikling af knock-out muskelcellelinjer ved hjælp af Lentivirus-medieret CRISPR / Cas9 genredigering
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Udvikling af knock-out muskelcellelinjer ved hjælp af Lentivirus-medieret CRISPR / Cas9 genredigering

Article DOI: 10.3791/64114-v • 10:12 min • June 16th, 2022
June 16th, 2022 •
1, 1, 1, 2, 2, 1, 1
1University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 2Genome Center, University of California, Davis

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Protokollen beskriver, hvordan man genererer knock-out myoblaster ved hjælp af CRISPR/Cas9, startende fra design af guide-RNA'er til den cellulære kloning og karakterisering af knock-out klonerne.

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Genetik udgave 184
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