Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Desenvolvimento de linhas de células musculares…
JoVE Journal
Genetics

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Genetics
Desenvolvimento de linhas de células musculares knock-out usando edição de genes CRISPR/Cas9 mediados por lentivírus
High-Resolution Comparison of Bacterial Conjugation Frequencies
Journal (Genetics)
High-Resolution Comparison of Bacterial Conjugation Frequencies
Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
Journal (Genetics)
Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
 
Click here for the English version

Desenvolvimento de linhas de células musculares knock-out usando edição de genes CRISPR/Cas9 mediados por lentivírus

Article DOI: 10.3791/64114-v • 10:12 min • June 16th, 2022
June 16th, 2022 •
1, 1, 1, 2, 2, 1, 1
1University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 2Genome Center, University of California, Davis

Chapitres

résumé
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

O protocolo descreve como gerar myoblasts knock-out usando CRISPR/Cas9, desde o design de guide-RNAs até a clonagem celular e caracterização dos clones knock-out.

Tags

Genética Edição 184
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter