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Genetics

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JoVE Journal Genetics
Utvikling av knock-out muskelcellelinjer ved hjelp av lentivirusmediert CRISPR / Cas9 genredigering
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
Journal (Genetics)
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
Journal (Genetics)
Parallel Measurement of Circadian Clock Gene Expression and Hormone Secretion in Human Primary Cell Cultures
 
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Utvikling av knock-out muskelcellelinjer ved hjelp av lentivirusmediert CRISPR / Cas9 genredigering

Article DOI: 10.3791/64114-v • 10:12 min • June 16th, 2022
June 16th, 2022 •
1, 1, 1, 2, 2, 1, 1
1University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, 2Genome Center, University of California, Davis

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Protokollen beskriver hvordan du genererer knock-out myoblasts ved hjelp av CRISPR / Cas9, fra utformingen av guide-RNAer til cellulær kloning og karakterisering av knock-out klonene.

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Genetikk utgave 184
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