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Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide
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Quantitative Determination of De Novo Fatty Acid Synthesis in Brown Adipose Tissue Using Deuterium Oxide

Article DOI: 10.3791/64219-v • 07:34 min • May 12th, 2023
May 12th, 2023 •
*1, *2, 1, 2,3,4
1UCD Conway Institute and UCD Institute of Food and Health, School of Agriculture and Food Science, University College Dublin, 2Division of Endocrinology, Cincinnati Children’s Hospital Medical Center, Cincinnati, 3Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, 4Department of Pediatrics, University of Cincinnati College of Medicine
* These authors contributed equally

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résumé
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Here, we present an inexpensive quantitative method utilizing deuterium oxide and gas chromatography mass spectrometry (GCMS) for the analysis of total fatty acid de novo lipogenesis in brown adipose tissue in vivo.

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Biology Brown Adipose Tissue Deuterium Oxide Metabolic Pathway Functional Roles Whole-body Metabolic Homeostasis Physiological Processes Pathological Processes Glucose Disposal Functional Assessment Detailed Protocols Quantitative Method Gas Chromatography Mass Spectrometry (GCMS) Total Fatty Acid De Novo Synthesis Carbon Source Sample Preparation Downstream Calculations Brown Fat Metabolic Homeostasis
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