Ex Vivo Intestines
Ex Vivo Intestines - Related Videos
12:00
Spatiotemporal Mapping of Motility in Ex Vivo Preparations of the Intestines
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Cited by 26 •
2016
Recently available video recording and spatiotemporal mapping (STmap) techniques make it possible to visualize and quantify both propagating and mixing patterns of intestinal motility. The goal of this protocol is to explain the generation and analysis of STmaps using the GastroIntestinal Motility Monitoring (GIMM) system.
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
06:04
Ex Vivo Intestinal Sacs to Assess Mucosal Permeability in Models of Gastrointestinal Disease
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Cited by 41 •
2016
This protocol describes the use of excised intestinal tissue preparations or "intestinal sacs" as an ex vivo model of intestinal barrier function. This model may be used to assess integrity of both the epithelial barrier and the mucous gel layer at specific intestinal sites in animal models of digestive disease.
09:09
The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids
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Cited by 4 •
2016
Here, a protocol to harvest, maintain, and treat mouse small intestinal organoids with pathogen associated molecular patterns (PAMPs) and Listeria monocytogenes is described, as well as emphasis on gene expression and proper normalization techniques for protein.
04:37
Intestinal Crypts Isolation: A Method to Isolate Whole Crypts from Small Intestine of Murine Model
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2023
In this video, we demonstrate a technique to isolate whole crypts from a mouse’s small intestine. The isolated whole crypts can be used to propagate long-lived and self-renewing 3D organoids in vitro.
FAQs
Maintaining appropriate nutrients, oxygen, and temperature supports tissue viability while experimental exposure is controlled outside the organism. This balance allows intestinal segments to retain aspects of native gut structure rather than reducing the investigation to isolated cellular behavior. Consequently, measurements of epithelial transport, barrier integrity, and secretion can be related to tissue-level function.
Experimental conditions determine how reliably the tissue reflects intestinal function. Nutrient and oxygen supply help sustain viable tissue, while temperature and the chosen exposure conditions shape responses to microbes, drugs, or dietary compounds. Researchers must therefore interpret transport, barrier, and secretion measurements in relation to the conditions used, not as context-free properties.
Unlike a cellular study, the model retains aspects of intestinal tissue structure; unlike a whole-animal experiment, it permits precise control over what reaches the tissue and when sampling occurs. This intermediate scale helps connect cell-level findings with whole-animal biology. It is especially useful when investigators need controlled exposure without losing all tissue-level responses.
Researchers obtain intestinal segments, maintain them under laboratory conditions that provide nutrients, oxygen, and suitable temperature, and then apply a defined experimental exposure. They can subsequently measure epithelial transport, barrier integrity, secretion, or responses to microbes, drugs, and dietary compounds. Tissue sampling during or after exposure supplies material for evaluating the experimental response.
The model can reveal changes in epithelial transport, barrier integrity, and secretion, as well as tissue responses to microbes, drugs, or dietary compounds. These readouts help investigators examine how intestinal tissue responds under controlled conditions. Because exposure and sampling are precise, the results can support comparisons among experimental treatments and link functional changes to the tested stimulus.
They support studies of digestion, intestinal disease, host-microbe interactions, and therapeutic delivery. The same approach can evaluate how dietary compounds or drugs affect intestinal tissue and can provide a controlled bridge between cellular experiments and whole-animal biology. It may also reduce reliance on animal experiments while allowing direct tissue sampling under defined conditions.