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JoVE Journal
Immunology and Infection

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The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids
 

The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids

Article doi: 10.3791/54033
May 18th, 2016

Summary May 18th, 2016

Here, a protocol to harvest, maintain, and treat mouse small intestinal organoids with pathogen associated molecular patterns (PAMPs) and Listeria monocytogenes is described, as well as emphasis on gene expression and proper normalization techniques for protein.

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